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Cleavage of supercoiled pUC19 DNA (0.5 μg) by the ligands and complexes (a) 1 and (b) 3 in a buffer containing 50 mM Tris-HCl at 37°C (30 min). lane M: marker; lane 1: DNA control; lane 2: L1 (10−3 M) + DNA; lane 3: DNA + H2O2; lane 4: L2 + DNA + H2O2; lane 5: complex (10−3 M) + DNA; lane 6: complex + DNA + H2O2.
