Figure 5.

uPAR-dependent matrigel invasion of chondrosarcoma. Cells human fibrosarcoma HT1080 and chondrosarcoma cells were subjected to cell invasion assays. Cells were preincubated with DMEM (none), 2 μg/mL indicated anti-uPAR antibodies, 10 nM RERF peptide or 10 nM ERFR peptide, plated in Boyden chambers and allowed to cross matrigel-coated filters for 18 h at 37°C in humidified air with 5% CO₂. 10% FBS (a), LB6, or LB6/hSuPAR conditioned media (b) were employed as chemoattractants. In all cases, data are reported as a percentage of invading cells in the absence of chemoattractant, considered as 100% (DMEM), and represent the average of two experiments, performed in duplicate. Columns, mean of two independent experiments; bars, ±SD, *P < .001 against the control (DMEM).