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. 2003 Dec 12;5:228–237. doi: 10.1251/bpo66

Fig. 3. Western blot analysis of the proteins synthesized by the ProteoLink IVEC system with ubiquitin antibody and specificity of UBA binding.

Fig. 3

A: Mixed protein pools. 96 protein pools were generated by employing TNT Quick Coupled in vitro transcription/translation system. The 96 protein pools were divided into 24 mixed protein pools and separated by SDS-PAGE, followed by immunoblotting with ubiquitin monoclonal antibody. B: Proteins synthesized in the IVEC system in the absence of ubiquitin (lane 1) or presence of wild type ubiquitin (lane 2), ubiquitin K29 (lane 3), ubiquitin K48 (lane 4), and ubiquitin K63 (lane 5). C: Proteins synthesized in the IVEC system were labeled by 35S methionine and used for pull down assays. Lane 1: proteins recovered with agarose beads alone; Lane 2: proteins recovered with p62 UBA agarose beads.