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. 2003 Dec 12;5:228–237. doi: 10.1251/bpo66

Fig. 4. Pull down assay of p62’s UBA domain with proteins (mixed protein pool) synthesized by IVEC system in the presence of [35S] methionine.

Fig. 4

The 24 mixed proteins pools were incubated with agarose-immobilised p62 UBA beads for 2 hours at 4ºC and washed three times in washing buffer. Bound proteins were released by boiling for 2 min in SDS-PAGE sample buffer and separated on 10% SDS-PAGE. Positive “hits” were marked by arrows and the positive protein pools were selected for subsequent deconvulation.