Fig. 5.

Model predictions following the addition of Ligand L versus modified synthetic data. The dashed-lines denote the mean simulated value over the ensemble while the gray region denotes the 95% confidence interval. The points denote the mean synthetic data used to validate the model. The validation data was generated from the training data by adding a background level of the ligand (L = 1) and by considering species not used for training (with the exception of protein 1). A: Cytosolic levels of protein 1 versus time. Points denote the O7 = (3,2,2,1) data set in the presence of background ligand (L = 1). B: Cytosolic levels of protein 2 versus time. Points denote the O8 = (3,2,2,2) data set in the presence of background ligand (L =1). C: Cytosolic levels of protein 3 versus time. Points denote the O8 = (3,2,2,2) data set in the presence of background ligand (L = 1). D: Cytosolic levels of mRNA for protein 2 versus time. Points denote the O8 = (3,2,2,2) data set in the presence of background ligand (L = 1).