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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Exp Neurol. 2010 Oct 21;227(1):149–158. doi: 10.1016/j.expneurol.2010.10.010

Figure 4. Intermediate-size and small dendrites with spiny appendages express intracellular and surface GluR2.

Figure 4

(A). Two dendritic profiles (GluR2-d1 and GluR1-d2) oriented in different planes of section express GluR2 labeling. A small dendritic process (GluR2-d1) with an apparent spine neck (asterisks) shows a cluster of immunogold particles for GluR2 (arrows) near a vesicular organelle (vo) just beneath the plasma membrane. Labeling for GluR2 is also present in a longitudinally sectioned dendritic profile (GluR2-d2). In this structure, immunogold particles (arrows) are affiliated with intracellular organelles, as well as non-synaptic areas of the plasma membrane. (B). A small dendritic profile (GluR2-d) with a spiny appendage (asterisks) expresses GluR2. Immunogold particles (arrows) are associated with the plasmalemma in the dendritic shaft, and a single gold particle is present in the spine neck (arrow). An asymmetric synaptic junction (arrowhead) is formed between an unlabeled axon terminal (ut) and the spine head (sp), which is devoid of GluR2 labeling. (C). The shaft of a small dendritic profile shows GluR2 labeling (arrows) on the plasma membrane distal from a spine neck (asterisks). An adjacent spine head (sp) contains a single gold particle beneath the postsynaptic density (arrowhead) of an asymmetric-type junction. Scale Bars= 0.5 μm.