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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Proteomics. 2010 Feb;10(3):520–531. doi: 10.1002/pmic.200900573

Figure 1.

Figure 1

Outline of protocol for the murine intranasal sensitization model. Endotoxin-free OVA and PM (UFP) were used to sensitize the animals (6 mice/group) for demonstration of the adjuvant effect of ambient UFP. On day 1, mice from the PM exposure group received 0.5 μg PM suspension intranasally. On day 2, endotoxin-free OVA (10 μg) was used together with PM (UFP) (0.5 μg) to sensitize the PM exposure group by intranasal instillation, which was repeated on days 4, 7 and 9. The OVA and control groups only received OVA and saline. All animals in the PM and OVA groups were subsequently challenged with 1% OVA aerosol on day 21 and 22.