Table 1.
Proteins with significant expression level changes in bronchoalveolar lavage fluid from mice challenged with OVA and OVA plus ultra fine particles
| Protein assigned | Spot No. | Accession Number | Mr (kDa) | Cal. pI | Sequence Coverage (%) | Mascot Score | Peptides Matched | Fold changed (OVA vs. PBS) | Fold changed (OVA/UFP vs. PBS) |
|---|---|---|---|---|---|---|---|---|---|
| Haptoglobin | 1 | HPT_MOUSE | 38,7 | 5.9 | 10 | 108 | 4 | +2.49 | +3.82 |
| 2 | 22 | 349 | 10 | -1.78 | -3.31 | ||||
| Polymeric-immunoglobulin receptor (Poly-Ig receptor) (PIGR) | 3 | PIGR_MOUSE | 84,9 | 5.3 | 19 | 511 | 15 | +5.77 | +6.77 |
| Thioredoxin (Trx) (ATL-derived factor) (ADF) | 4 | THIO_MOUSE | 11,7 | 4.8 | 20 | 64 | 2 | -1.55 | -2.81 |
| Coactosin-like protein | 5 | COTL1_MOUSE | 15.9 | 5.3 | 21 | 131 | 5 | +1.88 | +3.2 |
| Pulmonary surfactant-associated protein A (SP-A) | 6 | SFTPA_MOUSE | 26,1 | 5.3 | 14 | 173 | 2 | +1.94 | +2.52 |
| Ferritin light chain 1 | 7 | FRIL1_MOUSE | 20,8 | 5.7 | 22 | 238 | 4 | -1.64 | -1.95 |
| Chitinase 3-like protein 4 (Ym2) | 8 | CH3L4_MOUSE | 44,9 | 5.8 | 15 | 155 | 6 | +1.25 | +46.29 |
| Transcobalamin-2 | 9 | TCO2_MOUSE | 47,6 | 5.9 | 12 | 202 | 6 | +1.23 | +1.68 |
| Plasminogen | 10 | PLMN_MOUSE | 90,7 | 6.2 | 1 | 290 | 11 | -1.88 | -1.42 |
| Neutrophil gelatinase-associated lipocalin (NGAL) | 11 | NGAL_MOUSE | 22,9 | 9.0 | 13 | 132 | 9 | +5.24 | +5.50 |
| 12 | 13 | 76 | 6 | +5.69 | +5.25 | ||||
| Chitinase-3-like protein 1 (Cartilage glycoprotein 39) (GP-39) | 13 | CH3L1_MOUSE | 43 | 8.7 | 27 | 348 | 24 | +1.44 | +1.89 |
| Complement C3 (HSE-MSF) | 14 | CO3_MOUSE | 186,4 | 6.4 | 5 | 203 | 13 | +3.79 | +4.78 |
| 15 | 11 | 224 | 19 | +2.70 | +4.00 | ||||
| 16 | 4 | 173 | 7 | +37.61 | +83.33 | ||||
| 17 | 5 | 402 | 10 | +6.35 | +13.95 | ||||
| 18 | 5 | 146 | 6 | +5.33 | +6.95 | ||||
| Acidic mammalian chitinase | 19 | CHIA_MOUSE | 52 | 4.9 | 18 | 250 | 15 | +1.39 | +4.96 |
| 20 | 1 | 49 | 1 | +1.72 | +4.8 | ||||
| Serum albumin | 21 | ALBU_MOUSE | 68,6 | 5.8 | 9 | 124 | 5 | +8.97 | +17.04 |
| 22 | 15 | 224 | 11 | +1.49 | +4.14 | ||||
| Chitinase 3-like protein 3 (Ym1) | 23 | CH3L3_MOUSE | 44,4 | 5.4 | 25 | 274 | 13 | +1.49 | +4.38 |
| 24 | 27 | 282 | 14 | +5.89 | +20.15 | ||||
| 25 | 22 | 159 | 9 | +6.32 | +26.72 | ||||
| 26 | 30 | 368 | 22 | +2.34 | +5.27 | ||||
| Pulmonary surfactant-associated protein D (PSP-D) | 27 | SFTPD_MOUSE | 37,7 | 7.6 | 30 | 234 | 10 | +3.48 | +7.35 |
| 28 | 13 | 99 | 4 | +5.04 | +13.36 | ||||
| 29 | 18 | 250 | 9 | +2.60 | +4.27 | ||||
| 30 | 18 | 243 | 9 | +5.99 | +21.94 | ||||