Fig. 8.

hESCs secrete more protein than NSCs. (A) Proteins precipitated from the medium conditioned by I6 hESCs or NSCs for 10 hours were subjected to SDS-PAGE and stained with GelCode Coomassie Blue stain. Loading of precipitated material was corrected for the amount of cell protein in each experiment. Whole-cell lysate lanes are shown for 85 μg of protein. The control lane is from a medium- or Geltrex-only precipitation. The gels are representative of two independent experiments. (B) Quantification of total precipitated protein using densitometry. The intense band at 55 kDa, also visible in the control, was excluded from the quantification. Values are means+s.d. for three independent experiments. Significance between values was calculated using a Student's t-test. **P<0.01.