Fig. 4.

Integrin expression of subcell lines and inhibition of α5β1-integrin-mediated cell invasion. (A) Analysis by flow cytometry of both subclones revealed different α1, α2 and α6 integrin expression. One representative experiment out of six is shown. The corresponding bar graphs show MFI values (mean + s.e.m., n=6). (B) Flow cytometry analysis of integrin expression on α5β1^high cells after addition of unspecific control siRNA (top row) or specific siRNAs (bottom row) targeting integrins α5 (left), α1(middle) and α2 (right). One representative experiment out of at least three is shown. (C) Analysis by flow cytometry of α2β1^low and α2β1^high subclones. One representative experiment out of at least five is shown. The bar graphs show MFI values (mean + s.e.m., n=5). (D) Percentage of invasive cells and (E) invasion profiles of α5β1^high cells after addition of 100 μM of control antibodies (black) or blocking antibodies against integrins α5 (gray), β1 (orange), α1 (red) and α2 (blue). (F) Percentage of invasive cells and (G) invasion profiles of α5β1^high cells transfected with control siRNA (black) or with siRNAs targeting integrins α5 (gray), α1 (red) and α2 (blue). (H) Percentage of invasive cells and (I) invasion profiles of α2β1^low and α2β1^high subclones. *P<0.05, **P<0.01.