Fig. 5.
Effect of enzymatic ECM degradation and ERK1/2 inhibition on cell invasion. (A) Percentage of invasive α5β1high cells (red) was reduced after addition of a protease inhibitor cocktail (PI), whereas the number of invasive cells was increased in α5β1low cells (orange). (B) The invasion profile shows that the PI-treated α5β1high cells did not invade as deep as control cells (DMSO-treated), whereas the invasion profile was not altered in PI-treated α5β1low cells. (C–E) The percentage of invasive α5β1high cells (C) and their invasion depths (D) were decreased after addition of the inhibitors 328005 (30 μM; Erk-1), PD98059 (100 μM) or U0126 (100 μM), whereas the percentage of invasive α5β1low cells (C) was slightly reduced and their invasion depths (E) were only marginally altered. *P<0.05, **P<0.01, ***P<0.001.