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. 2011 Jan 11;124(3):405–413. doi: 10.1242/jcs.077503

Fig. 4.

Fig. 4.

Expression of human SGK1C or SGK1F isoforms in B-13 cells promotes the transdifferentiation of B-13 cells to B-13/H cells. B-13 cells cultured in six-well plates were transfected with 2 μg of the indicated plasmid construct [plus 2 μg of either Topflash (T) or Fopflash (F) combined with 0.02 μg of transfection control Renilla reporter vector] or treated with 10 nM DEX for 14 days to generate B-13/H cells. 14 days after transfection, cells were harvested and analysed by western blotting (A,C) or analysed for expression of luciferase activities (B,D). KD, kinase-dead. *P<0.05, compared with Topflash/RL-TK-transfected (i.e. no expression construct) B-13 cells using ANOVA (two-tailed).