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SGK1 can directly phosphorylate β-catenin. (A) B-13 cells cultured in six-well plates were transfected with 2 μg of the indicated plasmid construct or treated with 10 nM DEX for 14 days to generate B-13/H cells. After 14 days, cells were harvested and analysed for the indicated protein. (B) In vitro phosphorylation of β-catenin. Western blot for the indicated protein after incubation. Results presented are typical of three independent experiments.
