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Effect of changes in spinal acetylcholine levels on [3H]5-HT efflux. The effect of the AChE inhibitor neostigmine (Neo, 10 μM) with or without preincubation of a muscarinic antagonist [atropine (Atr) 1 μM] and the effect of AChE were compared to the control, SB, response. Statistical analysis according to one-way ANOVA followed by Dunnett's test for treatment vs. control comparisons: ***, P < 0.001.
