Fig. 1.

MCOLN1 interacts with the three members of the LAPTM family in yeast two-hybrid assays. A split-ubiquitin yeast two-hybrid assay was performed to corroborate the interaction of LAPTM proteins with MCOLN1. Interactions were tested by monitoring the growth of yeast cells expressing MCOLN1-Cub or MCOLN3-Cub bait with various Nub-LAPTM fusion proteins on agar plates lacking tryptophan and leucine (top), or tryptophan, leucine and histidine and containing 10 mM 3-aminotriazole (3AT; bottom). The vectors pAl-Alg5 and pDL2-Alg5 were used as positive and negative controls, respectively.