Fig. 2.

Physical interaction of MCOLN1 with the three members of the LAPTM family. (A,B) The interaction between MCOLN1 and LAPTM family members was tested by co-immunoprecipitation. HeLa cells were transfected with various combinations of the indicated vectors. FLAG-tagged MCOLN1 and MCOLN3 were immunoprecipitated using the anti-FLAG mAb and samples were assessed by western blotting using anti-FLAG antibody or anti-GFP antibody. The results shown are representative of three independent experiments. The predicted molecular weight is approximately 53.7 kDa for GFP–LAPTM4a, 62 kDa for GFP–LAPTM4b, 56.8 kDa for LAPTM5 and 66 kDa for MCOLN1-FLAG. Arrowheads indicate full-length proteins, arrows correspond to proteolytic fragments and asterisks represent oligomers.