Fig. 6.

Knockdown of syntaxin-5 impairs autophagic flux. (A,B) HeLa cells stably expressing the mRFP-GFP/LC3 construct were seeded on glass coverslips and transfected for 72 hours with 20 nM of either control or anti-syntaxin-5 siRNA. After 72 hours, cells were fixed and analysed by confocal microscope. 4× magnifications of the merged channels (insets from A) are shown in B. (C) The P values for assessing the number of LC3-II positive autophagosomes and autolysosomes were determined using Student's t-test (n=3; ***P<0.001; **P<0.01). Scale bars: 10 μm.