Figure 1. DNK_v4 Neurons Display Complete Elimination of I_A.

A, Shown are representative voltage-clamp recordings from a wild-type (wt) and DNK_v4 (DN) neuron in response to a voltage jump to +50 mV. Total whole-cell K⁺ current is elicited with a 500 ms prepulse of −125 mV (black trace). With a prepulse of −45 mV, K_v4 is completely inactivated and the total delayed rectifier current remains (grey trace). Insets show the K_v4 current trace obtained by subtracting the delayed rectifier current trace from the total K⁺ current trace. B, Average peak K_v4 and delayed rectifier (DR) currents shown for wt and DN neurons; there is no significant difference in amplitude of the delayed rectifier currents (DR) remaining wild-type and DNK_v4 neurons (N = 15 for each genotype). Scale bars represent 50 pA and 10 ms. C, Single embryonic neuron expressing the HA-tagged DNK_v4 subunit immunostained for HA. DNK_v4 appears to be localized to the cell body and in puncta along neuronal processes.