Figure 5. The ability of depletion of USP19 to regulate cell growth and p27^Kip1 is lost in FR3T3 fibroblasts transformed by constitutively active Ras.

(A) Rat FR3T3 fibroblasts expressing an activated Ras oncogene (Ras-V12) or empty vector were transfected with USP19 siRNA oligonucleotides #1, #43, or nonspecific control siRNA oligonucleotide (CTL). Forty-eight hours after transfection, cells were harvested and counted. Shown are means ± SE of triplicate samples. *, P<0.001 compared to CTL. (B) USP19 depleted FR3T3-vect or FR3T3-Ras cells from experiment described in Fig. 5A were harvested. Equal amounts of protein from lysates were analyzed by immunoblotting with the indicated antibodies.