Figure 3. Prediction of signal peptides in human DIA1 and DIA1R.

The amino acid sequence of (A) human DIA1 or (B) human DIA1R, was evaluated for amino-terminal signal peptides using the hidden Markov model of SignalP v3.0 [72]. C-region (cleavage-recognition region) scores are in aqua, H-region (hydrophobic region) scores are in dark blue, N-region (N-terminal signal peptide sequence) scores are in green, and the cleavage probability (that a given amino acid is the first in the mature protein) indicated in red. The significance cut-off value for all probability scores is 0.5, and is indicated by a blue dotted line. Standard single-letter amino acid abbreviations and numbering is provided below the graph.