Skip to main content
. 2011 Jan 17;6(1):e14526. doi: 10.1371/journal.pone.0014526

Figure 1. Genotyping PCR.

Figure 1

Offspring from mating between RT2/HRG+/− males and HRG+/− females were genotyped by a five-primer PCR. (A) The five primers give three distinct PCR products. Primer pair F-127/R+183 gives a 310 bp HRG wild type allele product, primer pair F-127/RNeo gives a 378 bp HRG knockout allele product and primer pair RT1/RT2 gives a 449 bp RT2 transgene allele product. The location of each primer is indicated in the map by an arrow. (B) PCR products separated on a 2% agarose gel. All six possible genotypes are presented.