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. 2011 Jan 17;6(1):e14526. doi: 10.1371/journal.pone.0014526

Figure 5. HRG regulates platelet levels in the circulation.

Figure 5

(A) Platelet numbers in whole blood from RT2 positive or negative mice with different HRG genotypes (HRG+/+ or HRG−/−). (B) Left panel; Quantification of the number of platelets/field in kidney tissue from healthy (RT2-negative) HRG+/+ or HRG−/− mice. Right panel; Quantification of the area percentage stained positive for the platelet marker CD41 in tumor tissue from RT2-positive HRG+/+ and HRG−/− mice. (C, D) Immunohistochemical staining directed against CD41 was performed on kidney from RT2-negative mice (C) and tumor tissue from RT2-positive mice (D) from both genotypes. Arrows indicate platelets. (E) HRG+/+ and HRG−/− mice were treated with Plavix (25 mg/ml or 50 mg/ml in drinking water) for three days. Platelet activation in whole blood was measured after stimulation with 10 µM ADP by flow cytometric analysis of the activated fibrinogen receptor (GPIIb/IIIa). (D) Platelet levels after Plavix treatment were measured by flow cytometric analysis in whole blood and are presented as the percentage of GPIX positive cells. Statistical analyses were performed with a two-tailed Mann-Whitney test, vertical bars represent standard deviation, * p≤0.05, ns = non significant. Scale bars represent 50 µm in C and 100 µm in D.