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. 2011 Jan 17;6(1):e16259. doi: 10.1371/journal.pone.0016259

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Niikura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, B. Brain homogenate of 3xTg-AD mice treated with S14G-HN (H) or vehicle (V) was subjected to immunoblot analysis using anti-phosphorylated tau (Ser202) (AT8) (upper panel in A), anti-phosphorylated tau (Thr231) (AT180) (upper panel in B), anti-total tau (tau46) (middle panels) and anti-beta-actin (lower panels) antibodies. C–F. Brain sections were subjected to immunostaining using anti-phosphorylated tau (Ser202) (AT8) antibody (C, D) or anti-phosphorylated tau (Thr231) (AT180) antibody (E, F) and biotin-conjugated secondary antibody followed by visualization with ABC method. Representative images of hippocampal CA1 region of vehicle (C, E) and S14G-HN (D, F) treated mouse sections are shown. Bar = 200 µm.