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. 2011 Jan 17;6(1):e16259. doi: 10.1371/journal.pone.0016259

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Niikura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A–D. Brain sections were subjected to immunostaining using anti-synaptophysin antibody followed by visualization with avidin-fluorescein. Representative images of hippocampal CA3 region of vehicle (A, C) and S14G-HN (B, D) treated mouse sections are shown. Bar = 20 µm E. Synaptophysin fluorescence intensity in 25 µmX80 µm area of the hippocampal CA3 region was measured. The relative value of S14G-HN-treated group (n = 3) to vehicle group (n = 3) is shown as mean±SD. P = indicates p value of t-test.