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. 2011 Jan 17;6(1):e16241. doi: 10.1371/journal.pone.0016241

Figure 2. TNFα and IFN-γ repress survivin and Bcl-xL expression in metastatic colon carcinoma cells.

Figure 2

A. Analysis of survivin and Bcl-xL protein and mRNA level. SW620 cells were treated with IFN-γ, TNFα or both IFN-γ and TNFα for 24 h. Cells were then analyzed by Western blotting analysis (top panel) for the level of the indicated proteins and by RT-PCR analysis (bottom panel) for mRNA level of the indicated genes. B. Analysis of protein levels of anti-apoptotic genes. Cells were treated as described in A and analyzed by Western blotting analysis for the indicated proteins. C. Caspase activation and apoptosis. SW620 cells were treated with IFN-γ, TNFα or both IFN-γ and TNFα overnight, followed by incubation with recombinant TRAIL protein (100 ng/ml) for the indicated time. Total cell lysates were then prepared and analyzed by Western blotting for activated caspase 8. Cytosol fractions were also prepared from cells as treated above and analyzed for cytochrome C release and PARP cleavage.