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. 2001 Feb 20;98(5):2808–2813. doi: 10.1073/pnas.051634198

Figure 1.

Figure 1

CaMK inhibition and MAPK inhibition have distinct effects on the time course of CREB dephosphorylation. (A) Depolarization (90 mM K+ for 3 min) induces rapid and stable CREB phosphorylation under control conditions in CA3/CA1 hippocampal pyramidal neurons. Cells are stained with anti-pCREB polyclonal antibody (green) and with anti-MAP2 monoclonal antibody (red) to identify neurons. Intensity histograms for pCREB are shown below each representative image. Note the all-or-none character of the pCREB staining during the recovery phase (wash 60′), as previously reported (7). (B) MEK inhibition with PD98059 (50 μM) selectively inhibits the late phase of CREB phosphorylation. (C) KN-93 (2 μM) abolishes the fast phase and reduces the slow phase of CREB phosphorylation. All results are representative of at least three independent experiments.