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. Author manuscript; available in PMC: 2012 Jan 27.
Published in final edited form as: Transplantation. 2011 Jan 27;91(2):137–145. doi: 10.1097/TP.0b013e3181ffff7d

Table 2.

Physical assays used to assess collagenase quality

Assay Ref Cost to
implement		 Advantages Disadvanatages
SDS-PAGE 72 Low

  • Low cost

  • Resolves intact and degraded forms of C1 and C2

  • Qualitative assessment of molecular forms

  • Subject to artifacts
Analytical anion exchange HPLC 66,69 Medium-high

  • Quantitative assessment

  • Resolves intact and degraded forms of C1

  • Peaks correlate with molecular form and specific CDA of enzyme

  • Rapid (< 60 min)

  • Requires specialized skill/equipment

  • Unable to resolve degraded forms of C2
Reversed phase HPLC 70,85 Medium-high

  • Quantitative

  • Requires specialized skill/equipment

  • Uses hazardous reagents

  • Unable to resolve intact and degraded forms of C1 or C2
Capillary electrophoresis 71 High

  • Quantitative

  • Unable to resolve C1b from C1c

  • Requires specialized skill/equipment