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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Exp Cell Res. 2010 Nov 24;317(4):392–404. doi: 10.1016/j.yexcr.2010.11.007

Figure 5. Cells that express the ErbB4 Q646C mutant display a higher degree of ErbB4 tyrosine phosphorylation and multimerization than do cells that express wild-type ErbB4.

Figure 5

MIA PaCa-2 cells were infected with recombinant adenoviruses that encode wild-type ErbB4 or the ErbB4 Q646C mutant. Controls included ψ2 mouse fibroblasts engineered to express the ErbB4 Q646C mutant or wild-type ErbB4. ErbB4 was immunoprecipitated and resolved using reducing or non-reducing polyacrylamide gels. The resolved samples were electroblotted onto PVDF. Blots were probed with an anti-phosphotyrosine antibody or an anti-ErbB4 antibody. The mobility and the reported molecular weight of the protein standards are indicated. The positions of ErbB4 monomers and multimers are also indicated.