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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Exp Cell Res. 2010 Nov 24;317(4):392–404. doi: 10.1016/j.yexcr.2010.11.007

Figure 7. Wild-type ErbB4 potentiates NRG1β stimulation of anchorage-independent growth of MIA PaCa-2 human pancreatic tumor cells.

Figure 7

These cells were infected with recombinant lentiviruses that carry the blasticidin resistance gene (Vector) or with lentiviruses that carry the blasticidin resistance gene along with wild-type ErbB4 (ErbB4) or the ErbB4 K751M mutant that lacks tyrosine kinase activity. Infected cells were selected using blasticidin and seeded in semisolid medium containing 10 nM NRG1β or PBS (diluent control). Following fifteen days of incubation, anchorage-independent colonies were photographed and colony diameter was measured by hand from the photographs. (A) Representative photomicrographs of cell lines cultured in the presence of NRG1β are depicted. (B) The effects of lentiviral infection and NRG1β stimulation on anchorage-independent proliferation were determined by using a Chi-square test to compare the colony size distribution for each experimental condition. The data represent five independent trials. P values are reported.