Fig. 3.

Dual control of transcription of oxidative stress regulon in C. glabrata. (A and B) Northern blot analysis of indicated transcripts during 0.4 mM H₂O₂ induced oxidative stress. C. glabrata wild type, Cgskn7Δ transformed with pCgSKN7 and Cgyap1Δ transformed with pCgYAP1 were grown to exponential phase before 0.4 mM H₂O₂ was added. (B) Wild type and Cgskn7Δyap1Δ were transformed with empty vector, pCgSKN7, pCgYAP1 or pCgYAP1-CgSKN7 (A). (C) Northern analysis of CgCTA1, CgSOD1 and CgSOD2 during glucose starvation. (D) Zone inhibition assay of Candida glabrata and S. cerevisiae strains with menadione (1.5 mM). 7 × 10⁶ cells were spread onto YPD. Plates were incubated at 30 °C for 24 h. Density dependent on the radial distance is indicated.