Figure 2.

miR-29b expression potently inhibits neuronal apoptosis induced by multiple stimuli. P3 sympathetic neurons were microinjected with miR-29b or cel-miR-67 (ctrl miRNA, each 30 μM) together with rhodamine to mark injected cells. (A,C,E) Forty-eight hours following injection, neurons were subjected to NGF deprivation (A), 20 μM etoposide (C), or 2.5 μM tunicamycin (E), and survival of injected cells was assessed at various time points following cell treatment. Survival was expressed as a percentage of viable cells prior to treatment. (B,D,F) Representative phase-contrast images of the exact field of sympathetic neurons before (untreated) or after 3 d of NGF deprivation (B), etoposide (D), or tunicamycin (F). Rhodamine marks cells injected with 30 μM miR-29b (arrows). Data in A, C, and E are mean ± SD of at least three independent experiments. Bar, 20 μm.