Skip to main content
. 2011 Jan 15;25(2):131–136. doi: 10.1101/gad.610511

Figure 2.

Figure 2.

Reorientation of Dachs with a new Dachsous gradient is sufficient to reorient tissue growth. (A) Diagram of the graded expression of dpp.Gal4, which is highest at the A–P compartment boundary and decays into the apical compartment. The dotted line shows the location of the region of interest. (B, top) Portion of a wild-type wing pouch showing oriented clones (βgal) along the P–D axis (vertical). A diagram of the endogenous gradient of Dachsous (Ds) in this portion of the wing pouch is shown to the left. (Bottom) Expression of Dachs-V5 using dpp.Gal4 shows polarization in the P–D axis. (C, top) Portion of a wing pouch expressing Dachsous (Ds) and GFP with dpp.Gal4 driver. Clones (βgal) orient according to the new Ds gradient. (Bottom) Expression of Dachs-V5 under dpp.Gal4 UAS.Ds shows polarization of Dachs along the new Ds gradient. (D) Quantification of spindle orientation in B (n = 70) versus C (n = 91). (E) Wild-type wing. (F) Pattern of dpp.Gal4 in the adult wing. (F′) A dpp.Gal4 UAS.Ds wing grows outward, perpendicular to the P–D axis, in the anterior compartment. (F″) Overlay with a wild-type wing. (G) Pattern of omb.Gal4 in the adult wing. (G′) An omb.Gal4 UAS.Ds wing grows outward, perpendicular to the P–D axis, in the both compartments. (G″) Overlay with a wild-type wing.