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. 2011 Jan 15;25(2):137–152. doi: 10.1101/gad.1980311

Figure 3.

Figure 3.

Crystal structure of the SUVH5 SRA domain bound to hemimethylated CG DNA. (A) Sequence of the complementary hemimethylated CG 10-mer containing a 5mC-G step on the top strand and an unmodified C-G step on the bottom strand in the center of the duplex. (B) Stick (DNA) and ribbon (protein) representation of the 2.37 Å crystal structure of the 2:1 SUVH5 SRA–hemimethylated CG DNA duplex complex. The color coding is the same as in Figure 2B. Note that the 5mC5 and the C5 from adjacent base pairs on partner strands flip out through the minor groove and are positioned in binding pockets of individual SRA domains. (C) Interaction of the looped-out C5 with residues lining the binding pocket in the structure of the complex. (D) Superposition of the flipped-out C5 (in green) and the flipped-out 5mC5 (in purple) within their respective binding pockets in the structure of the complex. (E) Insertion of anti 5mC5 (space-filling representation) into the binding pocket of the SRA domain (electrostatic surface presentation). (F) Insertion of anti C5 (space-filling representation) into the binding pocket of the SRA domain (electrostatic surface presentation).