FIGURE 2.

Sequences and predicted secondary structures of the small hairpin RNA substrates used in the study (left) and their pseudouridylation activity assay results (right). “TSL” is the abbreviation for T-stem–loop and “ACA” denotes the presence of ACA trinucleotide in the RNA. “CNG” stands for the Cbf5-Nop10-Gar1 ternary complex. The U55 is indicated by a star. The four major peaks are eluted standard nucleosides and their identities are labeled at the top (C for cytidine, U for uridine, G for guanosine, and A for adenosine). Pseudouridine is the earliest eluted peak and is labeled by Ψ.