FIGURE 4.

Expression of K01C8.6 and nduf-5 GFP fusion constructs in transgenic worms. Constructs in which the region upstream of nduf-5 or K01C8.6 was fused to the GFP gene are depicted above. For K01C8.6, 561 nt upstream of the trans-splice site along with the first exon of the gene were fused in frame to GFP. For nduf-5, the 578 nt upstream of the trans-splice site of the gene were fused to GFP. RNA was isolated from transgenic strains expressing these constructs and analyzed by RT-PCR. The mRNA is almost exclusively SL1 trans-spliced, indicating that the endogenous chromosomal context of nduf-5 and K01C8.6 is important in directing SL2 trans-splicing.