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. 2011 Feb;17(2):356–364. doi: 10.1261/rna.2393711

FIGURE 4.

FIGURE 4.

Cytosine in the position +4 [C(+4)] is required for RNase MRP cleavage (shown by an arrow); a replacement of C(+4) for any other base eliminates RNase MRP cleavage. (Crossed arrows) Positions of expected cleavage. (Lanes 1,6,11,16) Digest with RNase T1 (markers); (lanes 2,7,12,17) alkaline hydrolysis (markers); (lanes 3,8,13,18) untreated RNA substrates; (lanes 4,5) RNase MRP digest of a substrate containing 5′-UUUUACGC-3′ sequence; (lanes 9,10) RNase MRP digest of a substrate containing 5′-UUUUACGG-3′ sequence; (lanes 14,15) RNase MRP digest of a substrate containing 5′-UUUUACGA-3′ sequence; (lanes 19,20) RNase MRP digest of a substrate containing 5′-UUUUACGU-3′ sequence. Substrates were 5′-end-labeled with 32P and separated on a 6% denaturing polyacrylamide gel.