FIGURE 5.

Guanines flanking RNase MRP cleavage site reduce the efficiency of cleavage. (Lanes 1,6,11,16) Digest with RNase T1 (markers); (lanes 2,7,12,17) alkaline hydrolysis (markers); (lanes 3,8,13,18) untreated RNA substrates; (lanes 4,5) RNase MRP digest of the substrate 5′-GGN₁₉-UUUUAACUC-N₁₃-3′; (lanes 9,10) RNase MRP digest of the substrate 5′-GGN₁₉-UUUUAGCUC-N₁₃-3′; (lanes 14,15) RNase MRP digest of the substrate 5′-GGN₁₉-UUUUGACUC-N₁₃-3′; (lanes 19,20) RNase MRP digest of the substrate 5′-GGN₁₉-UUUUGGCUC-N₁₃-3′. Substrates were 5′-end-labeled with ³²P and separated on a 6% denaturing polyacrylamide gel.