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. Author manuscript; available in PMC: 2012 Mar 17.
Published in final edited form as: Behav Brain Res. 2010 Nov 17;218(1):29–41. doi: 10.1016/j.bbr.2010.11.026

Figure 1. Initial characterization of the MALTT transgenic line.

Figure 1

(a) A fluorescently labeled (FITC) anti-digoxigenin antibody indicated the location of the TyBS minigene insertion (left) and Geimsa staining indicated (black arrow) the labeled chromosome was X (right). (b) A schematic diagram of the mouse X chromosome indicates at minimum two copies of 4.4kb minigene construct (0.4kb MuLV sequence+1.8kb promoter region+2.2kb tyrosinase (tyr) cDNA) inserted in the non-coding region between genes Gm732 and Brwd3, as well as the 5’ integration sequence (black lettering: X chromosome, gray lettering:minigene). A Southern blot probe against the minigene is indicated in red. (c) A Southern hybridization image indicates 2 minigene positive bands (0.8kb, ~2.9kb) apparent in MALTT (lane 1) but not WT (lane 2) BanI-digested gDNA (left). (d) A single band (minimum 20kb) was apparent in MALTT (lane 1) but not WT (lane 2) BbsI-digested gDNA (right). (e) Exemplary sagittal and coronal Nissl-stained images of WT and MALTT brain sections are shown.