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All glial connexins can form functional homotypic channels in HeLa cells. Lentiviral transduction was used to generate lines expressing specific glial connexins. Immunostaining (A-E) reveals an orthodox distribution including puncta localized to apposing membranes of adjacent cells (Scale bar: 30 μm). HeLa cells expressing individual glial connexins were able to transfer neurobiotin (F-J), whereas an empty vector control was not (K). Scale bar: 50 μm.
