Table 1.
Predicted outcomes of Experimental treatments on the decline of kmax measurements in woody stems, assuming that the mechanism listed in the corresponding column header is operative (see Introduction for further explanations)
Experimental treatment | Non-steady-state flow | Pectin swelling | Bubbles | Active wounding response | Passive wounding effects |
Not degassed | o | o | o | o | o |
Flask-degassed at 30 kPa | o | o | + | o | o |
Degasser at 30 kPa | o | o | + | o | o |
Degasser at 3 kPa | o | o | ++ | o | o |
Hydrated at 3 kPa, degasser at 3 kPa | o | o | ++ | o | o |
Ice water | o | + | + | + | o |
Ice waterb | o | o | + | + | o |
Bark removed before cutting | o | o | o | + | + |
KCl, 10 mM | o | ++ | o | o | o |
KCl, 100 mM | o | ++ | + | o | o |
KCl, 300 mM | o | ++ | ++ | o | o |
CaCl2, 10 mM | o | ++ | o | o | o |
CaCl2, 40 mM | o | ++ | + | o | o |
CaCl2, 100 mM | o | ++ | ++ | o | o |
MgSO4, 0.5 mM | o | + | o | o | o |
MgSO4, 20 mM | o | ++ | + | o | o |
MgSO4, 50 mM | o | ++ | ++ | o | o |
(NH4)2SO4, 100 mM | o | o | ++ | o | o |
Cu(II)SO4, 0.1 mMb | o | o | o | + | o |
Cu(II)SO4, 0.25 mM | o | o | o | + | o |
4-HR, 10 mMb | o | o | o | + | o |
Hydroquinone, 10 mMb | o | o | o | + | o |
Degasser, in- and outflow measured | +a | o | + | o | o |
Symbols: o=no alleviation predicted; + =some alleviation predicted; ++ =strong alleviation predicted.
Experimental treatment accounts for, but does not alleviate, declines in kmax.
Treatment applied during vase rehydration and during measurement.