Table 1.
Experimental treatment | Non-steady-state flow | Pectin swelling | Bubbles | Active wounding response | Passive wounding effects |
Not degassed | o | o | o | o | o |
Flask-degassed at 30 kPa | o | o | + | o | o |
Degasser at 30 kPa | o | o | + | o | o |
Degasser at 3 kPa | o | o | ++ | o | o |
Hydrated at 3 kPa, degasser at 3 kPa | o | o | ++ | o | o |
Ice water | o | + | + | + | o |
Ice waterb | o | o | + | + | o |
Bark removed before cutting | o | o | o | + | + |
KCl, 10 mM | o | ++ | o | o | o |
KCl, 100 mM | o | ++ | + | o | o |
KCl, 300 mM | o | ++ | ++ | o | o |
CaCl2, 10 mM | o | ++ | o | o | o |
CaCl2, 40 mM | o | ++ | + | o | o |
CaCl2, 100 mM | o | ++ | ++ | o | o |
MgSO4, 0.5 mM | o | + | o | o | o |
MgSO4, 20 mM | o | ++ | + | o | o |
MgSO4, 50 mM | o | ++ | ++ | o | o |
(NH4)2SO4, 100 mM | o | o | ++ | o | o |
Cu(II)SO4, 0.1 mMb | o | o | o | + | o |
Cu(II)SO4, 0.25 mM | o | o | o | + | o |
4-HR, 10 mMb | o | o | o | + | o |
Hydroquinone, 10 mMb | o | o | o | + | o |
Degasser, in- and outflow measured | +a | o | + | o | o |
Symbols: o=no alleviation predicted; + =some alleviation predicted; ++ =strong alleviation predicted.
Experimental treatment accounts for, but does not alleviate, declines in kmax.
Treatment applied during vase rehydration and during measurement.