Fig. 4.

Analysis of protein contents during H. orientalis pollen tube growth. (A) Coomassie blue-stained gel of total proteins from pollen grains at maturity (MP), after hydration (H), and after 1, 3, 6, 12, 24, and 36 h of in vitro germination. Lanes marked as 6hCHX and 12hCHX indicate pollen tubes treated for 3 h with cycloheximide added to the medium at the third and ninth hour of growth, respectively. Lanes 6hC and 12hC indicate control pollen tubes growing for 6 h and 12 h, respectively. (B) Protein concentration (μg) in 1 μl of extracts isolated from the mature, hydrated, and germinating pollen grains of hyacinth. The temporal variants of pollen tube growth are the same as shown in A. Grey blocks of the graph correspond to the pollen tubes treated with cycloheximide. The values represent the average of three replicate experiments; the error bars indicate 1 SEM.