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. 2010 Nov 29;62(3):1325–1336. doi: 10.1093/jxb/erq376

Fig. 5.

Fig. 5.

Activity of recombinant VvGSTs in the in vitro synthesis of 3MH-S-glut. (A) The recombinant proteins VvGST1, VvGST3, and VvGST4, in SDS–PAGE. Escherichia coli sonicates are shown in the left panel. His-purified proteins (15 μl of purified solution) electrophoresed on a 12.5% SDS–polyacrylamide gel (right). M, protein marker. Asterisks indicate recombinant VvGST proteins. Vector, pCold I vector as negative control. (B) VvGST3 and VvGST4 mediate 3MH-S-glut synthesis in vitro. Data are shown as means ±SDs of triplicate experiments. n.d., not detected. CGST, commercial GST from equine liver.