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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Dev Cell. 2010 Dec 14;19(6):872–883. doi: 10.1016/j.devcel.2010.10.023

Figure 5. Light regulates GATA2 accumulation through a COP1 ubiquitin ligase-dependent process.

Figure 5

(A) Immunoblot analysis of GATA2 protein levels. Dark-grown (D) or light-grown (L or L/D) 5-day-old 35S:GATA2 transgenic seedlings (L3 line) were treated with mock solution (−) or 10 μM MG132 (+) for 4 hours in the dark (D and L/D) or light (L). Histone H3 was probed as a loading control. (B) Immunoblot assay of GATA2 protein level in 5-day old dark-grown wild type, the cop1 mutants, and cop1-4 crossed with the GATA-AS line. (C) In vitro pull-down assay showing the interaction between GATA2 and COP1. (D) In vitro ubiquitination assay showing ubiquitination of GST-GATA2 by MBP-COP1. The arrow points to the GST-GATA2 band and the star marks the ubiquitinated GST-GATA2 bands.