Figure 1.

SPARC deficiency stimulates osteolysis. RM1 cells (1 x 10³) were injected into the tibia of SPARC WT or KO mice. (A) Bones were isolated after 2 weeks of intraosseous growth, sectioned, and stained with H&E. RM1 cells (T) can be seen growing in the proximal metaphysis and have completely replaced the bone marrow. B indicates trabecular bone; GP, growth plate. Scale bars, 50 µm. (B) MicroCT-derived transaxial slices from mice 1 day (initial) and 2 weeks (final) after tumor implantation. Severe osteolysis can be seen in KO mice. Arrow indicates site of cortical breach. Representative images from nine mice are shown.