Figure 6.
SPARC KO osteoclasts are hyperresorptive. BMMΦs were isolated from the bone marrow of SPARC WT (WT cells, black columns) or KO (KO cells, white columns) mice. Marrow was flushed and plated in the presence of 100 ng/ml M-CSF. After 3 days, 5 x 105 BMMΦ cells were then placed on parietal bones from either SPARC WT (WT bone) or KO (KO bone) mice and treated with 30 ng/ml M-CSF and 100 ng/ml RANKL for 5 more days. (A) Pits formed by osteoclasts were visualized microscopically. Scale bars, 50 µm. Representative images from 10 fields are shown. (B) Pit area was measured and represented as mean percentage area resorbed ± SD (n = 10 fields from duplicate experiments). **P < .01 and ***P <.001 by one-way ANOVA.