Figure 3.

In vivo injection of β-NEU1-neutralizing antiserum inhibits the normal development of K_Ca in CG neurons. (A) β-NEU1-neutralizing antiserum, saline vehicle, or nonimmune serum were injected into the brains of E9 chick embryos. Density of ionic currents was determined by whole-cell recordings at E11, a stage at which K_Ca density is normally increased in CG neurons (Upper). CG neurons from embryos injected with β-NEU1-neutralizing antiserum exhibited significantly reduced mean K_Ca density compared to neurons from embryos injected with nonimmune serum (Lower). (B) In contrast, injection of neutralizing antiserum had no effect on voltage-activated Ca²⁺ currents in CG neurons.