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. Author manuscript; available in PMC: 2011 Jan 18.
Published in final edited form as: Langmuir. 2009 Sep 1;25(17):10038–10044. doi: 10.1021/la900966h

Figure 3. Bond dissociation constants (koff) are lowered by increasing the length of the HuEP-PEG spacer on the microparticle.

Figure 3

Distributions of the lifetimes of the transient adhesions were used to calculate the bond dissociation constants (koff) of chemisorbed, PEG3400, and PEG10000 linked HuEP microparticles interacting with the P-selectin surface at wall shear stresses of 0.1 dynes/cm2, 0.4 dynes/cm2, and 1.0 dynes/cm2. The relationship between koff and wall shear rate was determined for (grey) chemisorbed, (black) PEG3400 and (white) PEG10000 linked microparticles. The P- selectin surface site density was 100 sites/µm2. R2 > 0.94 for the bi-molecular reaction model equation: ln[C] = −koff t + constant. 26,37 Dissociation constant differences that achieve significance are indicated by asterisk (* p<0.01) for HuEP3400 koff versus chemisorbed HuEP and (** p<.01) for HuEP10000 koff versus chemisorbed HuEP koff.