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. 2011 Jan 18;9(1):e1000581. doi: 10.1371/journal.pbio.1000581

Figure 2. Schematic for in silico sorting of the SecM RNCs.

Figure 2

The unsorted volume (A) containing a total of 1.1 million particles with density in all three tRNA binding sites was initially sorted into two populations (B) based on the ratchet-like subunit rearrangement of the small subunit relative to the large subunit. The ratcheted population (350,000 particles; 32%) had tRNAs present in A/P- and P/E-sites, whereas the unratcheted population (750,000 particles; 68%) could be further sorted into three subpopulations (C): a dominant fraction (544,000 particles; 73%) with P-tRNA only, and two minor fractions with A- and P-tRNAs (65,000; 12%) and with P- and E-tRNAs (40,000; 7%).