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. 2010 Dec 16;10:88. doi: 10.1186/1472-6750-10-88

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Copyright ©2010 Liu and Kearney; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of p19 on GFP expression of FECT/GFP in trans and cis formats in N. benthamiana. GFP fluorescence at 2 dpi as seen under UV light for the standard 35S-driven FECT40/GFP co-agroinoculated with 35S/GFP (trans) or for a binary vector containing both 35S/FECT40/GFP and Pnos-driven p19 driven (cis). The cis format was constructed in an attempt to better visualize FECT40/GFP expression in plants with poor rates of agroinoculation (such as monocots), to eliminate the need for double infection by both p19 and FECT40/GFP.